First Iberian R1b-DF27 sample, probably from incoming East Bell Beakers


I had some more time to read the paper by Valdiosera et al. (2018) and its supplementary material.

One of the main issues since the publication of Olalde et al. (2018) (and its hundreds of Bell Beaker samples) was the lack of a clear Y-DNA R1b-DF27 subclades among East Bell Beaker migrants, which left us wondering when the subclade entered the Iberian Peninsula, since it could have (theoretically) happened from the Chalcolithic to the Iron Age.

My prediction was that this lineage found today widespread among the Iberian population crossed the Pyrenees quite early, during the Chalcolithic, with migrating East Bell Beakers expanding North-West Indo-European dialects, and that it spread slowly afterwards.

The first ancient sample clearly identified as of R1b-DF27 subclade is found in this paper, at the Late Bronze Age site Cueva de los Lagos. Although it is unidentified and has no radiocarbon date, the site as a whole is associated with the Cogotas culture and its Bouquique ceramic decoration.

Y-DNA and mtDNA haplogroups, from the paper. Sequencing statistics and contamination rates for newly generated sequence data.

It was found in the northern part of the Cogotas culture territory (which lies mainly between Castille and Aragon, in North-Central Spain), shows evident steppe admixture, and it has become obvious with the latest papers (including this one) that R1b-M269 lineages intruded south of the Pyrenees associated with East Bell Beaker migrations.

The Proto-Cogotas culture is associated with a Bell Beaker substrate influenced by either El Argar or Atlantic Bronze, and the specific type of ceramics found at this Cogotas culture site are probably from the mid-2nd millennium, which is too early for the Celtic expansion.

Supervised ADMIXTURE results.

Nevertheless, due to the quite likely late date of the sample (in the centuries around 1500 BC), there is still a possibility that incoming R1b-DF27 lineages were not among the early R1b-M269 lineages found in the Iberian Chalcolithic, and were associated with later migrations from Central Europe, potentially linked to the expansion of the Urnfield culture, and thus nearer to an Italo-Celtic community.

Diachronic map of migrations in Europe ca. 1250-750 BC.

In any of these scenarios, a Pre-Celtic expansion of North-West Indo-European in Iberia (possibly associated with Lusitanian) is still the best explanation for the origin and expansion of (at least some) modern Iberian R1b-DF27 lineages, including those found among the Basque-speaking population.

This implies that the ‘indigenous’ Neolithic lineages of Iberia (like I2 and G2a2) were replaced with subsequent internal gene flows and founder effects, such as those that evidently happened (probably quite recently) among Basques, even though indigenous languages show an obvious continuity.

I would say this is the last nail in the coffin for autochthonous Y-DNA continuity theories for Spain and France (i.e. for the traditional Vasconic-Uralic hypothesis), but we know that data is never enough for any die hard continuist…so let’s just say another nail in the coffin for endless autochthonous continuity theories.

EDIT (18/3/2018): Genetiker has published Y-SNP calls for both R1b samples, showing this one is R1b1a1a2a1a2a-BY15964 (see modern members of this subclade in ytree), and that the other one is R1b1a1a2a~L23.


Patterns of genetic differentiation and the footprints of historical migrations in the Iberian Peninsula

Open access preprint (which I announced already) at bioRxiv Patterns of genetic differentiation and the footprints of historical migrations in the Iberian Peninsula, by Bycroft et al. (2018).

Abstract (emphasis mine):

Genetic differences within or between human populations (population structure) has been studied using a variety of approaches over many years. Recently there has been an increasing focus on studying genetic differentiation at fine geographic scales, such as within countries. Identifying such structure allows the study of recent population history, and identifies the potential for confounding in association studies, particularly when testing rare, often recently arisen variants. The Iberian Peninsula is linguistically diverse, has a complex demographic history, and is unique among European regions in having a centuries-long period of Muslim rule. Previous genetic studies of Spain have examined either a small fraction of the genome or only a few Spanish regions. Thus, the overall pattern of fine-scale population structure within Spain remains uncharacterised. Here we analyse genome-wide genotyping array data for 1,413 Spanish individuals sampled from all regions of Spain. We identify extensive fine-scale structure, down to unprecedented scales, smaller than 10 Km in some places. We observe a major axis of genetic differentiation that runs from east to west of the peninsula. In contrast, we observe remarkable genetic similarity in the north-south direction, and evidence of historical north-south population movement. Finally, without making particular prior assumptions about source populations, we show that modern Spanish people have regionally varying fractions of ancestry from a group most similar to modern north Moroccans. The north African ancestry results from an admixture event, which we date to 860 – 1120 CE, corresponding to the early half of Muslim rule. Our results indicate that it is possible to discern clear genetic impacts of the Muslim conquest and population movements associated with the subsequent Reconquista.

“(a) Binary tree showing the inferred hierarchical relationships between clusters. The colours and points correspond to each cluster as shown on the map, and the length of the coloured rectangles is proportional to the number of individuals assigned to that cluster. We combined some small clusters (Methods) and the thick black branches indicate the clades of the tree that we visualise in the map. We have labeled clusters according to the approximate location of most of their members, but geographic data was not used in the inference. (b) Each individual is represented by a point placed at (or close to) the centroid of their grandparents’ birthplaces. On this map we only show the individuals for whom all four grandparents were born within 80km of their average birthplace, although the data for all individuals were used in the fineSTRUCTURE inference. The background is coloured according to the spatial densities of each cluster at the level of the tree where there are 14 clusters (see Methods). The colour and symbol of each point corresponds to the cluster the individual was assigned to at a lower level of the tree, as shown in (a). The labels and boundaries of Spain’s Autonomous Communities are also shown.”

Some interesting excerpts:

Our results further imply that north west African-like DNA predominated in the migration. Moreover, admixture mainly, and perhaps almost exclusively, occurred within the earlier half of the period of Muslim rule. Within Spain, north African ancestry occurs in all groups, although levels are low in the Basque region and in a region corresponding closely to the 14th-century ‘Crown of Aragon’. Therefore, although genetically distinct this implies that the Basques have not been completely isolated from the rest of Spain over the past 1300 years.

NOTE. I must add here that the Expulsion of Moriscos is known to have been quite successful in the old Crown of Aragon – deeply affecting its economy – , in contrast with other territories of the Crown of Castille, where they either formed less sizeable communities, or were dispersed and eventually Christened and integrated with local communities. For example, thousands of Moriscos from Granada were dispersed following the War of Alpujarras (1567–1571) into different regions of the Crown of Castille, and many could not be later expelled due to the locals’ resistance to follow the expulsion edict.

Perhaps surprisingly, north African ancestry does not reflect proximity to north Africa, or even regions under more extended Muslim control. The highest amounts of north African ancestry found within Iberia are in the west (11%) including in Galicia, despite the fact that the region of Galicia as it is defined today (north of the Miño river), was never under Muslim rule and Berber settlements north of the Douro river were abandoned by. This observation is consistent with previous work using Y-chromosome data. We speculate that the pattern we see is driven by later internal migratory flows, such as between Portugal and Galicia, and this would also explain why Galicia and Portugal show indistinguishable ancestry sharing with non-Spanish groups more generally. Alternatively, it might be that these patterns reflect regional differences in patterns of settlement and integration with local peoples of north African immigrants themselves, or varying extents of the large-scale expulsion of Muslim people, which occurred post-Reconquista and especially in towns and cities.

We estimated ancestry profiles for each point on a fine spatial grid across Spain (Methods). Gray crosses show
the locations of sampled individuals used in the estimation. Map shows the fraction contributed from the donor group ‘NorthMorocco’.

Overall, the pattern of genetic differentiation we observe in Spain reflects the linguistic and geopolitical boundaries present around the end of the time of Muslim rule in Spain, suggesting this period has had a significant and long-term impact on the genetic structure observed in modern Spain, over 500 years later. In the case of the UK, similar geopolitical correspondence was seen, but to a different period in the past (around 600 CE). Noticeably, in these two cases, country-specific historical events rather than geographic barriers seem to drive overall patterns of population structure. The observation that fine-scale structure evolves at different rates in different places could be explained if observed patterns tend to reflect those at the ends of periods of significant past upheaval, such as the end of Muslim rule in Spain, and the end of the Anglo-Saxon and Danish Viking invasions in the UK.

Certain people want to believe (well into the 21st century) into ideal ancestral populations and ancient ethnolinguistic identifications linked to one’s own – or the own country’s dominant – ancestral components and Y-DNA haplogroup.

We are nevertheless seeing how mainly the most recent relevant geopolitical events and late internal migratory flows have shaped the genetic structure (including Y-DNA haplogroup composition) of modern regions and countries regardless of its population’s actual language or ethnic identification, whether (pre)historical or modern.

Another surprise for many, I guess.


Population substructure in Iberia, highest in the north-west territory (to appear in Nature)

A manuscript co-authored by Angel Carracedo, from the University of Santiago de Compostela, and (always according to him) pre-accepted in Nature, will offer more insight into the population substructure of Spain, based on autosomal DNA.

Carracedo’s lecture about DNA (in Galician), including his summary of the paper (from december 2017):

Some of the points made in the video:

  • The study shows a situation parallelling – as expected – the expansion of Spanish Medieval kingdoms during the Reconquista (and subsequent repopulation).
  • In it, the biggest surprise seems to be the greater substructure found in Galicia, the north-western Spanish territory – greater even than expected by the authors.
  • As a side note, Galicia shows a great influence from Moorish” ancestral components, due mainly to the influx from Portugal, which shows more.

It is difficult to judge only from the image and his words, but one could say that there are:

  • Certain quite old ancestral Galician groups;
    • then two – also quite old – ancestral Basque groups;
      • then more recent Galician groups;
        • and then a common, central Spanish group – including
          • a wider Asturian-Catalan group, with a western Asturian-Leonese, and an eastern Catalan subgroup;
          • and a central Castillian-Aragonese group, also with a western Castillian, and an eastern Aragonese subgroup.
Spain’s population substructure, from the video.

We thought that certain parts of the British Isles could show ancestral components related to the old population, although this has not proven exactly right, due to more recent population expansions.

However, this paper might shed light to the controversy surrounding Lusitanian (possibly Gallaico-Lusitanian) as a Pre-Celtic Indo-European group of Iberia, either slightly older as an Italo-Celtic dialect, or potentially from the Bell Beaker expansion, whose genetic imprint might have survived the Roman conquest, which apparently didn’t replace its ancestral population.

Given the presence of a central Spanish group opposed to the other minor groups – and knowing that (at least part of) the Medieval kingdoms should be related to the Occitan region – due to the Celtic expansion, and also potentially later during the Visigothic Kingdom, and the Carolingian Empire – , we can only guess that the other (north-western and Basque) groups are potentially quite old, and reflect prehistoric population structures.

Just speculating here, of course. Another interesting genetic paper to await…

Seen first in the Facebook group Iberia ADN.


Asian ancestry of the Roma people in Europe

New article, Tau haplotypes support the Asian ancestry of the Roma population settled in the Basque Country, by Alfonso-Sánchez et al., Nature (2017).


We examined tau haplotype frequencies in two different ethnical groups from the Basque Country (BC): Roma people and residents of European ancestry (general population). In addition, we analyzed the spatial distribution of tau haplotypes in Eurasian populations to explore the genetic affinities of the Romani groups living in Europe in a broader scope. The 17q21.31 genomic region was characterized through the genotyping of two diagnostic single nucleotide polymorphisms, SNPs (rs10514879 and rs199451), which allow the identification of H1 and H2 haplotypes. A significant heterozygous deficit was detected in the Romani for rs10514879. The H2 haplotype frequency proved to be more than twice in the BC general population (0.283) than in the Roma people (0.127). In contrast, H2 frequency proved to be very similar between Basque and Hungarian Romani, and similar to the H2 frequencies found in northwestern India and Pakistan as well. Several statistical analyses unveiled genetic structuring for the MAPT diversity, mirrored in a significant association between geography and genetic distances, with an upward trend of H2 haplotype frequencies from Asia to Europe. Yet, Roma samples did not fit into this general spatial patterning because of their discrepancy between geographical position and H2 frequency. Despite the long spatial coexistence in the Basque region between the residents of European ancestry and the Roma, the latter have preserved their Asian genetic ancestry. Bearing in mind the lack of geographical barriers between both ethnical groups, these findings support the notion that sociocultural mores might promote assortative matings in human populations.

“Regression line and 95% confidence intervals (dashed lines) in a regression analysis of tau H2 haplotype frequencies on the rotated geographical coordinates (H2 freq = 0.4256 − coord × 0.000083) of 35 European and Asian populations (coefficient of determination, r2 = 0.515). Populations examined in this study are highlighted with a frame. Solid circles are European populations, solid squares are Middle Eastern populations, and solid triangles represent South Asian populations. Romani populations are designated by stars. Population labels: BCRoma (Basque Country Roma), BC-resid (Basque Country general population), BC-Spain (Iberian Basques), BC-French (French Basques), UK (British), IT-Sardn (Sardinia, Italy), ITBergm (Bergamo, Italy), ITBresc (Brescia, Italy), IT-Tuscn (Tuscany, Italy), HU-Roma (Hungarian Roma), Palestn(Palestinians), and Samartn (Samaritans)”

I just realized I forgot to include the migration of Indo-Aryan Roma people in the map of medieval migrations… I shall correct that in future versions.

Map showing the migrations of Romani people through Europe and Asia minor. From Wikipedia.

Featured image: Map of Romani dialects. From Wikipedia, by ArnoldPlaton.

Analysis of R1b-DF27 haplogroups in modern populations adds new information that contrasts with ‘steppe admixture’ results


New open access article published in Scientific Reports, Analysis of the R1b-DF27 haplogroup shows that a large fraction of Iberian Y-chromosome lineages originated recently in situ, by Solé-Morata et al. (2017).


Haplogroup R1b-M269 comprises most Western European Y chromosomes; of its main branches, R1b-DF27 is by far the least known, and it appears to be highly prevalent only in Iberia. We have genotyped 1072 R1b-DF27 chromosomes for six additional SNPs and 17 Y-STRs in population samples from Spain, Portugal and France in order to further characterize this lineage and, in particular, to ascertain the time and place where it originated, as well as its subsequent dynamics. We found that R1b-DF27 is present in frequencies ~40% in Iberian populations and up to 70% in Basques, but it drops quickly to 6–20% in France. Overall, the age of R1b-DF27 is estimated at ~4,200 years ago, at the transition between the Neolithic and the Bronze Age, when the Y chromosome landscape of W Europe was thoroughly remodeled. In spite of its high frequency in Basques, Y-STR internal diversity of R1b-DF27 is lower there, and results in more recent age estimates; NE Iberia is the most likely place of origin of DF27. Subhaplogroup frequencies within R1b-DF27 are geographically structured, and show domains that are reminiscent of the pre-Roman Celtic/Iberian division, or of the medieval Christian kingdoms.

Some people like to say that Y-DNA haplogroup analysis, or phylogeography in general, is of no use anymore (especially modern phylogeography), and they are content to see how ‘steppe admixture’ was (or even is) distributed in Europe to draw conclusions about ancient languages and their expansion. With each new paper, we are seeing the advantages of analysing ancient and modern haplogroups in ascertaining population movements.

Quite recently there was a suggestion based on steppe admixture that Basque-speaking Iberians resisted the invasion from the steppe. Observing the results of this article (dates of expansion and demographic data) we see a clear expansion of Y-DNA haplogroups precisely by the time of Bell Beaker expansion from the east. Y-DNA haplogroups of ancient samples from Portugal point exactly to the same conclusion.

The situation of R1b-DF27 in Basques, as I have pointed out elsewhere, is probably then similar to the genetic drift of Finns, mainly of N1c lineages, speaking today a Uralic language that expaned with Corded Ware and R1a subclades.

The recent article on Mycenaean and Minoan genetics also showed that, when it comes to Europe, most of the demographic patterns we see in admixture are reminiscent of the previous situation, only rarely can we see a clear change in admixture (which would mean an important, sudden replacement of the previous population).

Equating the so-called steppe admixture with Indo-European languages is wrong. Period.

The following are excerpts from the article (emphasis is mine):

Dates and expansions

The average STR variance of DF27 and each subhaplogroup is presented in Suppl. Table 2. As expected, internal diversity was higher in the deeper, older branches of the phylogeny. If the same diversity was divided by population, the most salient finding is that native Basques (Table 2) have a lower diversity than other populations, which contrasts with the fact that DF27 is notably more frequent in Basques than elsewhere in Iberia (Suppl. Table 1). Diversity can also be measured as pairwise differences distributions (Fig. 5). The distribution of mean pairwise differences within Z195 sits practically on top of that of DF27; L176.2 and Z220 have similar distributions, as M167 and Z278 have as well; finally, M153 shows the lowest pairwise distribution values. This pattern is likely to reflect the respective ages of the haplogroups, which we have estimated by a modified, weighted version of the ρ statistic (see Methods).

Z195 seems to have appeared almost simultaneously within DF27, since its estimated age is actually older (4570 ± 140 ya). Of the two branches stemming from Z195, L176.2 seems to be slightly younger than Z220 (2960 ± 230 ya vs. 3320 ± 200 ya), although the confidence intervals slightly overlap. M167 is clearly younger, at 2600 ± 250 ya, a similar age to that of Z278 (2740 ± 270 ya). Finally, M153 is estimated to have appeared just 1930 ± 470 ya.

Haplogroup ages can also be estimated within each population, although they should be interpreted with caution (see Discussion). For the whole of DF27, (Table 3), the highest estimate was in Aragon (4530 ± 700 ya), and the lowest in France (3430 ± 520 ya); it was 3930 ± 310 ya in Basques. Z195 was apparently oldest in Catalonia (4580 ± 240 ya), and with France (3450 ± 269 ya) and the Basques (3260 ± 198 ya) having lower estimates. On the contrary, in the Z220 branch, the oldest estimates appear in North-Central Spain (3720 ± 313 ya for Z220, 3420 ± 349 ya for Z278). The Basques always produce lower estimates, even for M153, which is almost absent elsewhere.

Simplified phylogenetic tree of the R1b-M269 haplogroup. SNPs in italics were not analyzed in this manuscript.


The median value for Tstart has been estimated at 103 generations (Table 4), with a 95% highest probability density (HPD) range of 50–287 generations; effective population size increased from 131 (95% HPD: 100–370) to 72,811 (95% HPD: 52,522–95,334). Considering patrilineal generation times of 30–35 years, our results indicate that R1b-DF27 started its expansion ~3,000–3,500 ya, shortly after its TMRCA.

As a reference, we applied the same analysis to the whole of R1b-S116, as well as to other common haplogroups such as G2a, I2, and J2a. Interestingly, all four haplogroups showed clear evidence of an expansion (p > 0.99 in all cases), all of them starting at the same time, ~50 generations ago (Table 4), and with similar estimated initial and final populations. Thus, these four haplogroups point to a common population expansion, even though I2 (TMRCA, weighted ρ, 7,800 ya) and J2a (TMRCA, 5,500 ya) are older than R1b-DF27. It is worth noting that the expansion of these haplogroups happened after the TMRCA of R1b-DF27.

Principal component analysis of STR haplotypes. (a) Colored by subhaplogroup, (b) colored by population. Larger squares represent subhaplogroup or population centroids.

Sum up and discussion

We have characterized the geographical distribution and phylogenetic structure of haplogroup R1b-DF27 in W. Europe, particularly in Iberia, where it reaches its highest frequencies (40–70%). The age of this haplogroup appears clear: with independent samples (our samples vs. the 1000 genome project dataset) and independent methods (variation in 15 STRs vs. whole Y-chromosome sequences), the age of R1b-DF27 is firmly grounded around 4000–4500 ya, which coincides with the population upheaval in W. Europe at the transition between the Neolithic and the Bronze Age. Before this period, R1b-M269 was rare in the ancient DNA record, and during it the current frequencies were rapidly reached. It is also one of the haplogroups (along with its daughter clades, R1b-U106 and R1b-S116) with a sequence structure that shows signs of a population explosion or burst. STR diversity in our dataset is much more compatible with population growth than with stationarity, as shown by the ABC results, but, contrary to other haplogroups such as the whole of R1b-S116, G2a, I2 or J2a, the start of this growth is closer to the TMRCA of the haplogroup. Although the median time for the start of the expansion is older in R1b-DF27 than in other haplogroups, and could suggest the action of a different demographic process, all HPD intervals broadly overlap, and thus, a common demographic history may have affected the whole of the Y chromosome diversity in Iberia. The HPD intervals encompass a broad timeframe, and could reflect the post-Neolithic population expansions from the Bronze Age to the Roman Empire.

While when R1b-DF27 appeared seems clear, where it originated may be more difficult to pinpoint. If we extrapolated directly from haplogroup frequencies, then R1b-DF27 would have originated in the Basque Country; however, for R1b-DF27 and most of its subhaplogroups, internal diversity measures and age estimates are lower in Basques than in any other population. Then, the high frequencies of R1b-DF27 among Basques could be better explained by drift rather than by a local origin (except for the case of M153; see below), which could also have decreased the internal diversity of R1b-DF27 among Basques. An origin of R1b-DF27 outside the Iberian Peninsula could also be contemplated, and could mirror the external origin of R1b-M269, even if it reaches there its highest frequencies. However, the search for an external origin would be limited to France and Great Britain; R1b-DF27 seems to be rare or absent elsewhere: Y-STR data are available only for France, and point to a lower diversity and more recent ages than in Iberia (Table 3). Unlike in Basques, drift in a traditionally closed population seems an unlikely explanation for this pattern, and therefore, it does not seem probable that R1b-DF27 originated in France. Then, a local origin in Iberia seems the most plausible hypothesis. Within Iberia, Aragon shows the highest diversity and age estimates for R1b-DF27, Z195, and the L176.2 branch, although, given the small sample size, any conclusion should be taken cautiously. On the contrary, Z220 and Z278 are estimated to be older in North Central Spain (N Castile, Cantabria and Asturias). Finally, M153 is almost restricted to the Basque Country: it is rarely present at frequencies >1% elsewhere in Spain (although see the cases of Alacant, Andalusia and Madrid, Suppl. Table 1), and it was found at higher frequencies (10–17%) in several Basque regions; a local origin seems plausible, but, given the scarcity of M153 chromosomes outside of the Basque Country, the diversity and age values cannot be compared.

Within its range, R1b-DF27 shows same geographical differentiation: Western Iberia (particularly, Asturias and Portugal), with low frequencies of R1b-Z195 derived chromosomes and relatively high values of R1b-DF27* (xZ195); North Central Spain is characterized by relatively high frequencies of the Z220 branch compared to the L176.2 branch; the latter is more abundant in Eastern Iberia. Taken together, these observations seem to match the East-West patterning that has occurred at least twice in the history of Iberia: i) in pre-Roman times, with Celtic-speaking peoples occupying the center and west of the Iberian Peninsula, while the non-Indoeuropean eponymous Iberians settled the Mediterranean coast and hinterland; and ii) in the Middle Ages, when Christian kingdoms in the North expanded gradually southwards and occupied territories held by Muslim fiefs.

Contour maps of the derived allele frequencies of the SNPs analyzed in this manuscript. Population abbreviations as in Table 1. Maps were drawn with SURFER v. 12 (Golden Software, Golden CO, USA).

I wouldn’t trust the absence of R1b-DF27 outside France as a proof that its origin must be in Western Europe – especially since we have ancient DNA, and that assertion might prove quite wrong – but aside from that the article seems solid in its analysis of modern populations.


Text and figures from the article, licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit

Neolithic and Bronze Age Basque-speaking Iberians resisted invaders from the steppe


Good clickbait, right? I have received reports about this new paper in Google Now the whole weekend, and their descriptions are getting worse each day.

The original title of the article published in PLOS Genetics (already known by its preprint in BioRxiv) was The population genomics of archaeological transition in west Iberia: Investigation of ancient substructure using imputation and haplotype-based methods, by Martiniano et al. (2017).

Maybe the title was not attractive enough, so they sent the following summary, entitled “Bronze Age Iberia received fewer Steppe invaders than the rest of Europe” (also in From their article, the only short reference to the linguistic situation of Iberia (as a trial to sum up potential consequences of the genetic data obtained):

Iberia is unusual in harbouring a surviving pre-Indo-European language, Euskera, and inscription evidence at the dawn of history suggests that pre-Indo-European speech prevailed over a majority of its eastern territory with Celtic-related language emerging in the west. Our results showing that predominantly Anatolian-derived ancestry in the Neolithic extended to the Atlantic edge strengthen the suggestion that Euskara is unlikely to be a Mesolithic remnant. Also our observed definite, but limited, Bronze Age influx resonates with the incomplete Indo-European linguistic conversion on the peninsula, although there are subsequent genetic changes in Iberia and defining a horizon for language shift is not yet possible. This contrasts with northern Europe which both lacks evidence for earlier language strata and experienced a more profound Bronze Age migration.

Judging from the article, more precise summaries of potential consequences would have been “Proto-Basque and Proto-Iberian peoples derived from Neolithic farmers, not Mesolithic or Palaeolithic hunter-gatherers”, or “incomplete Indo-European linguistic conversion of the Iberian Peninsula” – both aspects, by the way, are already known. That would have been quite unromantic, though.

Their carefully selected title has been unsurprisingly distorted at least as “Ancient DNA Reveals Why the Iberian Peninsula Is So Unique“, and “Ancient Iberians resisted Steppe invasions better than the rest of Europe 6,000 years ago“.

So I thought, what the hell, let’s go with the tide. Using the published dataset, I have also helped reconstruct the original phenotype of Bronze Age Iberians, and this is how our Iberian ancestors probably looked like:

Typical Iberian village during the Steppe invasion, according to my phenotype study of Martiniano et al. (2017). Notice typical invaders to the right.

And, by the way, they spoke Basque, the oldest language. Period.

Now, for those new to the article, we already knew that there is less “steppe admixture” in Iberian samples from southern Portugal after the time of east Bell Beaker expansion.

(A) PCA estimated from the CHROMOPAINTER coancestry matrix of 67 ancient samples ranging from the Paleolithic to the Anglo-Saxon period. The samples belonging to each one of the 19 populations identified with fineSTRUCTURE are connected by a dashed line. Samples are placed geographically in 3 panels (with random jitter for visual purposes): (B) Hunter-gatherers; (C) Neolithic Farmers (including Ötzi) and (D) Copper Age to Anglo-Saxon samples. The Portuguese Bronze Age samples (D, labelled in red) formed a distinct population (Portuguese_BronzeAge), while the Middle and Late Neolithic samples from Portugal clustered with Spanish, Irish and Scandinavian Neolithic farmers, which are termed “Atlantic_Neolithic” (C, in green).

However, there is also a clear a discontinuity in Neolithic Y-DNA haplogroups (to R1b-P312 haplogroups). That means obviously a male-driven invasion, from the North-West Indo-European-speaking Bell Beaker culture – which in turn did not have much “steppe admixture” compared to other north-eastern cultures, like the Corded Ware culture, probably unrelated to Indo-European languages.

Summary of the samples sequenced in the present study.

As always, trying to equate steppe or Yamna admixture with invasion or language is plainly wrong. Doing it with few samples, and with the wrong assumptions of what “steppe admixture” means, well…

Proto-Basque and Proto-Iberian no doubt survived the Indo-European Bell Beaker migrations, but if Y-DNA lineages were replaced already by the Bronze Age in southern Portugal, there is little reason to support an increased “resistance” of Iberians to Bell Beaker invaders compared to other marginal regions of Europe (relative to the core Yamna expansion in eastern and central Europe).

As you know, Aquitanian (the likely ancestor of Basque) and Iberian were just two of the many non-Indo-European languages spoken in Europe at the dawn of historical records, so to speak about Iberia as radically different than Italy, Greece, Northern Britain, Scandinavia, or Eastern Europe, is reminiscent of the racism (or, more exactly, xenophobia) that is hidden behind romantic views certain people have of their genetic ancestry.

Some groups formed by a majority of R1b-DF27 lineages, now prevalent in Iberia, spoke probably Iberian languages during the Iron Age in north and eastern Iberia, before their acculturation during the expansion of Celtic-speaking peoples, and later during the expansion of Rome, when most of them eventually spoke Latin. In Mediaeval times, these lineages probably expanded Romance languages southward during the Reconquista.

Before speaking Iberian languages, R1b-DF27 lineages (or older R1b-P312) were probably Indo-European speakers who expanded with the Bell Beaker culture from the lower Danube – in turn created by the interaction of Yamna with Proto-Bell Beaker cultures, and adopted probably the native Proto-Basque and Proto-Iberian languages (or possibly the ancestor of both) near the Pyrenees, either by acculturation, or because some elite invaders expanded successfully (their Y-DNA haplogroup) over the general population, for generations.

Maybe some kind of genetic bottleneck happened, that expanded previously not widespread lineages, as with N1c subclades in Finland.

There is nothing wrong with hypothetic models of ancient genetic prehistory: there are still too many potential scenarios for the expansion of haplogroup R1b-DF27 in Iberia. But, please, stop supporting romantic pictures of ethnolinguistic continuity for modern populations. It’s embarrassing.

Featured image from Wikipedia, and Pinterest, with copyright from Albert Uderzo and publisher company Hachette.

Images from the article, licensed CC-by-sa, as all articles from PLOS.

Rhetoric of debates, discussions and arguments: Useful destructive criticism for scientific & academic research, reasons and personal opinions; the example of Proto-Indo-European language revival

Rhetoric (Wikipedia) is the art of harnessing reason, emotions and authority, through language, with a view to persuade an audience and, by persuading, to convince this audience to act, to pass judgement or to identify with given values. The word derives from PIE root wer-, ‘speak’, as in MIE zero-grade wrdhom, ‘word’, or full-grade werdhom, ‘verb’; from wrētōr ρήτωρ (rhētōr), “orator” [built like e.g. wistōr (<*widtor), Gk. ἵστωρ (histōr), “a wise man, one who knows right, a judge” (from which ‘history’), from PIE root weid-, ‘see, know’]; from that noun is adj. wrētorikós, Gk. ρητορικός (rhētorikós), “oratorical, skilled in speaking”, and fem. wrētorikā, GK ρητορική (rhētorikē). According to Plato, rhetoric is the “art of enchanting the soul”.

When related to Proto-Indo-European language revival, as well as in modern scientific research of any discipline, discussions are sometimes interesting in light of historical rhetoric, as they might get really close to some classical (counter-)argumentative resources, however unknown they are to their users…

Sophists taught that every argument could be countered with an opposing argument, that an argument’s effectiveness derived from how “likely” it appeared to the audience (its probability of seeming true), and that any probability argument could be countered with an inverted probability argument. Thus, if it seemed likely that a strong, poor man were guilty of robbing a rich, weak man, the strong poor man could argue, on the contrary, that this very likelihood (that he would be a suspect) makes it unlikely that he committed the crime, since he would most likely be apprehended for the crime. They also taught and were known for their ability to make the weaker (or worse) argument the stronger (or better).

So, for example, if people might generally think that evolution is very likely to have occured, because of the scientifical data available, one only has to say something like “God put those proofs there to confound people and prove their faith“. And, even if there is no single reason to give why that person is entitled to interpret the Bible that way, and to determine what ‘God thought’ when ‘inventing proofs of a false evolution’, in fact there is no need to give rational arguments: this very likelihood of evolution is in itself a proof of how good God is in cheating us…

Statistics was a discipline mostly unknown to sophists, but I’m sure they more or less imagined the typical bell curve that population beliefs and opinions follow. If interpreted the other way round, one could say that the more an idea is believed by people, the more likely is that someone will come along with another, competing one. In fact, that’s natural evolution, too: without that universal trend that life has to differentiate itself from the normal, matter would have never changed and get more and more complicated…

That trend is observed in research, too, as man is obviously another animal and its intelligence another natural feature subjected to the evolutive machinery of nature. That’s why Occam’s razor is never a sufficient argument to end a research field or hypothesis: you have e.g. Gimbutas’ theories (or Renfrew’s, if you like) – even though obviously not completely proven hypothesis -, about some prehistoric speakers being successful in their conquests and migrations through Eurasia, which infers with logic that what happend with Indo-European languages expansion is what has almost always happened in the known history of language expansion, using the most probable extrapolation they can with the facts we know. But you will still find competing hypothesis about an unlikely millennium-long, peaceful spread and mix of languages through and from Europe or Asia, based on some controversial facts and a great part of imagination. And, even if such theories are far away from what can generally be considered rational, they will certainly find supporters; and it’s not bad that such unlikely ideas emerge: science is built up thanks to some of such marginal ideas which eventually prove true; apart from the million ones that prove false and disappear, and some dozens that are sadly able to remain, like homeopathy or Esperanto-like conlanging, as I’ve said before. The same happens with the human body, which went through mutation obtaining lots of advantages, but at the same time dragging some genetic illnesses along…

About Proto-Indo-European research, it’s more or less straightforward which hypothesis and theories are considered generally accepted, and which ones minority views. Nevertheless, that doesn’t prevent renown experts from accepting some marginal hypothesis in some aspects of PIE reconstruction, while keeping the general view on other ones; neither does that prevent renown linguists and philologists to consider Proto-Indo-European, or comparative and historical grammar in general, an absurd work: the ex-Dean of a southern Spanish University, a Latin professor, deems PIE an “invention”; in his words, “from Lat. pater, Gk. pater, and Eng. father, we say there is a language that said what, ‘pater‘? pfff”; he obviously considers “language=written & renown language system”; the problem with that thought is that if PIE becomes spoken (i.e. written too) and renown, just as Old Latin became Classical Latin – instead of disappearing as the other Italic dialects – the whole reasoning is useless; so it’s also useless now. One of the most famous Indo-Europeanists in Spain, F. Adrados (e.g. marginal supporter of Etruscan as an IE language) and Bernabé (e.g. marginal supporter of the Glottalic theory, I think), even if dedicated to Indo-European reconstruction, deemed PIE revival – in some news in Spanish newspaper El Mundo – a “uthopia“, but considered at the same time possible that Greek and Latin (respectively) became EU’s official language: it’s not that they don’t consider speaking PIE impossible, but only that there are “better” alternatives: better, I guess, for Romance or Greek speakers or philologists…

About Proto-Indo-European language revival for Europe, thus, it is difficult to ascertain if it is the most rational choice, as it is to ascertain if liberal thoughts are more rational than conservative ones. I have lived in other countries within the European Union, and have visited other parts of Spain where the spoken language is not Spanish; from that experience, the different attitudes I’ve found are overwhelming: when you speak in English or German anywhere in Europe, the conversation is everything but fluent; also, if you speak English in the UK, German in Germany, French in France, or Czech in Czechia, even mastering quite well the regional language, you’ll never get the same reaction as if a Catalan (from a Catalan-speaking region) speaks Spanish in, say, Galicia (a Galician-Portuguese speaking region), as both use a language (Spanish) common to both of them. That was also the idea behind the first Esperanto out there, probably Volapük, and it has been the idea behind every conlang trying to be THE International Auxiliary Language since then; and none has succeeded. That was also the idea behind Hebrew revival in Israel, for speakers of a hundred different languages living in the same territory: they had other modern, common languages to choose instead of an ancient, partially incomplete, and “difficult” (in Esperantist terms) one, too, and it succeeded.

Latin use in Europe, on the other hand, has been declining ever since the first Romance dialects developed, and had its latest offcial (i.e. legal) use in Europe, apart from the Catholic church, at the beginning of the XX century in Hungary – curiously enough, a non-Indo-European speaking country. Its revival has been proposed a thousand times since then, but has never recovered its prestige, as Germanic-speaking countries have taken the lead in Western Europe, and Slavic-speaking countries in the East. It is hard to explain now why English- or German- or Polish-speaking peoples should learn and speak again the language of the Romans and the Roman Empire, with which they have little history in common…

The rest of known language revivals, like Cornish or Manx, or even e.g. the partial revival (“sociolect”) of Katharevousa Greek, not to talk about the so-called “revivals” – in fact “language revitalizations” – of Basque, Catalan, Breton, Ukrainian, etc. have been just regionally oriented language (or prestige + vocabulary) revivals with cultural or social purposes.

So, is Proto-Indo-European revival a “correct”, or “sufficiently rational” option, given the known facts? As an opinion, it is neither correct nor incorrect, as being “Indo-Europeanist for Europe” is like being leftist or conservative in politics; just like supporting Hebrew revival wasn’t (a hundred years ago) “sufficiently rational” in itself, and controversy over its revival have never ended. But, the reasons behind PIE revival can and should be questioned, as the reasons behind a conlang adoption (i.e. the concepts of “better” and “easier” when applied to language) can and should be critically reviewed. In Proto-Indo-European, it refers – I think – to two main questions:

1) Did Proto-Indo-European exist? i.e. can we confidently consider any proto-language something different from especulation or mere unproven hypothesis? The answer is “it depends”. Proto-Indo-European was probably a language spoken by prehistorical people, as probable as any generally accepted scientific theory we can support without experimental proofs, like theories on the Universe, its creation or development: they might prove wrong in the future, but – following the necessary abstraction and common sense – it’s not difficult to accept most individual premises and facts surrounding them. That migh be said about proto-languages like Proto-Slavic (ca. 1 AD), Proto-Germanic (ca. 1000 BC), Proto-Greek or Proto-Indo-Iranian (ca. 2000 BC) or Proto-Indo-European, especially about its European or North-Western subbranch (ca. 2500-2000 BC); on the other hand, however, about proto-languages like ‘Proto-Eurasiatic’ or ‘Proto-Nostratic’, or ‘Proto-Indo-Tyrrhenian’, or ‘Proto-Thraco-Illyrian’, or ‘Proto-Indo-Uralic’, or ‘Proto-Italo-Celtic’ (or even Proto-Italic), or ‘Proto-Balto-Slavic’, and the hundred other proposed combinations, it is impossible to prove beyond doubt if and when they were languages at all.

2) Is the Proto-Indo-European reconstruction trustable enough to be “revived”? i.e. can we consider it a speakable language, or just a linguistic theoretical approach? Again, it depends, but here mostly mixed with political opinions. In light of Ancient Hebrew – a language that ceased to be spoken 2500 years ago -, “revived” as a modern language introducing thousands of newly coined terms – many of them from Indo-European origin -, to the point that some want to name it “Israeli”, instead of “Hebrew” (as we call MIE “European” or “Europaio” instead of “Indo-European”), I guess the answer is clearly yes, it’s possible: in any possible case, Indo-European languages have a continuated history of more than 4000 years, and modern terms need only (in most cases) a sound-law adjustment to be translated into PIE. Also, in light of the other proto-languages with a high scientifical basis and a similar time span, like Proto-Uralic, Proto-Semitic or Proto-Dravidian, there is no possible comparison with Proto-Indo-European: while PIE is practically a fully reconstructed and well-known language without written texts to ‘confirm’ our knowledge, the rest are just experimental (mainly vocabulary-based) reconstructions. There are, thus, proto-languages and proto-languages, as there are well-known natural dead languages and poorly attested ones; PIE is therefore one of the few ones which might be called today a real, natural language, like Proto-Germanic, Proto-Slavic or Proto-Indo-Aryan.

However, anti-Europeanists (or, better, anti-Indo-Europeanists for the European Union) won’t find it difficult to say a simple “a proto-language is not enough to be revived, as Ancient Hebrew was written down and PIE wasn’t”, thus disguising their sceptic views on the politics behind the project with seemingly rational discussion. While others will also state, in light of our clear confrontation with conlangs, that “proto-language is nothing different from a conlang”, thus disguising their real interest in spreading their personal desire that a proto-language be similar to a conlang. One only has to say: “Classical Latin couldn’t be reconstructed by comparing Spanish, French and Italian” – when, in fact, the question should be something like “could the common, Late Vulgar Latin, be reconstructed with a high degree of confidence, having just the writings of the first mediaeval romance languages?” The answer is probably a simple “yes,and quite well”, until proven the contrary, but by expressing the first doubt one can easily transform the possible-reconstruction argument in an apparently unlikely one; enough to convince those who want to be convinced…

Thus, whereas some people consider PIE a natural language, confidently reconstructed, but impossible to speak today because of political matters, others just consider it another invention, nothing different from Esperanto, while Esperantist talk about it as a “worse” or “more difficult” alternative to it: you could nevertheless find all opinions mixed together when it comes to destructive discussions, as the objective is not to defend an own rational and worked idea, but simply to destroy the appearance (or likelihood, in sophistic terms) of the rival’s idea. Be it anti-Europeanism, anti-Indo-European-reconstrution or anti-everything-else-than-Esperanto, you don’t have to defend your position: just repeat your known anti- cliches, and you’ve “won”. Apparently, at least.

Cicero noted what Greek rhetors already knew before about usual debates, and how arguments should be made and countered so that no idea is left accepted. In that sense, discussions were (and are) generally so unnecessary, that the Socratic Method seems to be still the best philosophical approach to discussions, even those concerning scientifical (i.e. “most probable”) facts: Instead of arriving at answers, non-expert (and often expert) discussion is used to break down the theories others hold, not “to go beyond the axioms and postulates we take for granted” and obtain a better knowledge, as Greek philosophers put it, but just to destroy what others build up.

So, for example, we might get these general rules to counter any argument, even if it’s not only based on opinions, but also on generally accepted facts:

1) Demonstrate the falseness of a part of the rival’s argument; then, infer the falseness of the whole reasoning. For example, let’s say Gimbutas’ view is out-dated, or that we at Dnghu included something considered nowadays ‘wrong’ in our grammar: then PIE revival is also mistaken; nothing more to explain. Or, let’s say that Hebrew revival is not “equal” to a proto-language revival, and that therefore the comparison is ‘false’ – even if comparisons are there to compare similar cases, not “equal” cases, which would be absurd – then, the whole PIE revival project is ‘equivocal’ or ‘absurd’. That’s the view about PIE revival you can find in some comments made on American blogs out there.

2) You can also confirm a part of your rival’s argument, and then, by doing it, carry that argument to its extreme, to the extent that the consequences of it are intolerable, and the paroxism completely distorts your rival’s argument. That’s more or less what I usually do when confronting conlanging as a real option for the European Union, by saying “OK, let’s adopt the ‘better’ and ‘easier’ language: first Esperanto, then the “better” and “easier” Esperanzo, then Lojban, then Pilosofio, then Mazematio, etc. etc. ad infinitum” – so, as a conclusion, one might accept that “better” and “easier” are not actually good reasons to adopt a language; hence the arguments based on “better” and “easier” cliches are opinion, not ratio.

3) The most common now (and then, I guess, in spoken language) is personal discredit, by which you can infer that his argument is also corrupted. That is what some have made when lacking more arguments, calling me personally (and the Indo-European language Association in general ?!) a “racist”, “nazi”, or “KKK-like” group; or trying to discredit me personally by saying I don’t master the English language; or that I misspelled or ‘was wrong’ in reconstructing this or that PIE name or noun; or even just because I am “an amateur”, – thus suggesting we all have to be “language professionals” to propose a trustable PIE revival. A recent example of this is our latest Esperantist visitor, saying I am “close to being racist” because I propose PIE for the EU – thus obviously inviting readers to identify “language=race”, saying that “I propose one language = I propose one race = I am a racist”, and therefore if “I=racist” and “I propose PIE revival” => “PIE=x”. The whole reasoning is nonsense, but he is not the first – and won’t be the last – educated individual to say (and possibly believe) that…

4) The fourth is actually only a minor method derived from the third, used in desperate cases, which consists on taking a sensible, emotional example of the consequences of the generalization of the rival’s argument, to demonstrate the moral baseness of the one who defends it; then, if he is discredited, his argument is corrupted, too [see point 3]… That is what some desperate people do when saying that PIE revival for the EU is “bad” (or “worse”) for non-IE-language-speakers like Finnish, Hungarian, Estonian, Basque or Maltese peoples. In fact, anyone who had taken a look at our website, or had made a quick search about me, would have found that I began this project of PIE revival to defend European languages (at least minority languages, as national or official languages are already well protected) against the European Union’s English officious imperium and English-German-French official triumvirate. Also, if we left PIE revival, only some languages (the official, i.e. national ones, 25 today) would get EU support, while the rest just die out or resist with some regional or private support. With Modern Indo-European, on the other hand, there will only be one official language supported by the European Union, and the rest really equal in front of each other and the Union, be it English, Maltese, Basque, Saami or Piedmontese. Nowadays, English is the language spoken in institutions, Maltese has an official status before the EU, while Saami is official in its country, Basque is only official in its territory, and Piedmontese, Asturian, Breton, and the majority of EU regional languages are only privately and locally defended. Nevertheless, one only has to say “supporting Indo-European is what Nazis did, PIE revival is racist and wants to destroy non-Indo-European peoples and cultures”; and, there you are: nothing proven, nothing reasoned, but the simplest and most efficient FUD you can find to counter the thousand arguments in favour of this revival project.

However unnecessary and unfruitful it might seem, I still discuss – or even directly look for debate -, because I get a benefit of such long, active pauses from my study, unlike those tiny passive TV- or radio-pauses I insert between study hours, especially in these stressful exam periods. Indeed I can find something to discuss in any website at any time, but I’m generally interested in debating these language political options. Nevertheless, I find it difficult to understand why some people get mad (at me, the project, or even the association or the whole world), when in fact taking part on any discussion is freely accepted by all of us, and it’s me who put new ideas and proposals on the table, and the others who just have to criticize them…

Something valuable for life I learned from psychology (possibly the only thing…) is about Chomsky’s reaction on Skinner’s comments: my professor (close to Freudian psychoanalysis), who told us the story – I hope I got it well, I cannot find it out there – thought it was Skinner who “won” the debate, by answering to Chomsky’s criticism, who in turn had criticized Skinner’s work, Verbal Behaviour, for his “scientistic”, not scientific, concept of the human mind. In fact, the younger Chomsky had just applied science to psychology (a need that psychology still has), simplifying the understanding of mind with a strict cognitive view, and criticizing some traditional views that psychologists accepted as ‘normal’. Skinner and those who followed his behavioural school of thought overreacted, mostly based on the belief that Chomsky’s reasons were against their lives and professional options, when in fact reason and opinion are in different planes. Chomsky, instead of entering the flame (yes, trolling existed back in the 60’s) did nothing. When asked years later, about why he didn’t reply as expected to all that criticism, he just said: “they missed the point”; he said what he had to say, criticized what he wanted, proposed an alternative, and left the discussion. And still, even by not answering, cognitive revolution provoked a shift in American psychology between the 1950s through the 1970s from being primarily behavioral to being primarily cognitive.

If you want to debate about opinions – be it PIE revival, Europeanism, general politics, Star Trek or the sex of angels -, entering into unending criticisms and personal attacks, that’s OK; but you should do it if and when you want, as I only do it because I obtain something beneficial, having a good time, laughing a little bit, relaxing from study, thinking about interesting reasons that might appear for or against my views or ideas, etc. And you should do it to get something in (re)turn, be it that same stress relief I (and most people) get, or other personal or professional benefits whatsoever. If not, if maybe you are getting more stressed trying to “convince” me or others, to “make us change our minds” with great one-minute ‘reasons’, by discussing directly your opinions as if they were ‘true‘, then you are clearly “missing the point” (using Chomsky’s words) with these discussions, and – as our latest Esperantist commenter (Mr. Janoski) puts it – “losing your time”, “trying to understand” something…